Acquired brain injury (ABI) is a complex disease that involves loss of brain functions related to cognitive and motor capabilities and that can produce unilateral spatial neglect (USN). The heterogeneity of the symptoms of these disorders causes a lack of consensus on suitable tools for evaluation and treatment. Recently, several studies have initiated the application of virtual reality (VR) systems as an evaluation instrument for neuropsychological disorders. Our main objective was to evaluate the validity of the VR Street Crossing Test (VRSCT) as an assessment tool. Twenty-five patients with ABI were evaluated with traditional tests and with the VRSCT. The results showed significant correlations between the conventional tests and the measures obtained with the VRSCT in non-negligent patients. Moreover, the VRSCT indicated significant differences in performance of negligent and non-negligent subjects. These pilot results indicate that ABI patients with and without USN can be assessed by the therapists using the VRSCT system as a complementary tool.