A fuzzy c-means clustering algorithm was used to assign soil nutrient to management zones which was based on remote sensing as data source in Nongwushi 81 Tuan Xin Jiang drip irrigation in cotton based on GIS and RS. The results showed that the variation coefficient of nutrient index was decreased in management zones based on remote sensing data source, space distribution were all the same direction. There were no significant differences among the three management zones. The space variation of soil nutrient content was different lowest in the same management zone. The conformity degree of the integration of management zones based on remote sensing NDVI as data was reached 75.47%. A fuzzy c-means clustering algorithm which was based on remote sensing as data source can achieve good management zones results, which could be used to help guide the rate of variable inputs and precise fertilizer application and provide the theory basis of soil nutrient management in cotton.