Knowledge representation and use are fundamental processes in many areas. The use of a semantic referential (i.e, a domain ontology and a set of related tools to exploit it) to represent knowledge has allowed the development of new mechanisms of semantic search, inferences, and analysis of complex content, but the development of a semantic referential is still a complex task, time-consuming and fundamentally performed by knowledge holders. Taking that into account this work discusses the development of a semantic referential applied to botanical identification process in the Brazilian Amazon area, mainly focused on the mechanisms of interaction and access to a domain ontology (named Onto-AmazonTimber) based on JENA API and SPARQL queries. The main aspects of the development of this work are presented and discussed here. Current challenges and open points are also addressed.