It is well known that the stochastic nature of the interference deeply impacts on the performance of emerging and future wireless communication systems. In this work we consider an ad hoc network where the mobile nodes adopt the Random Waypoint mobility model. Assuming a time-varying wireless channel due to slow and fast fading and, considering the dynamic path loss caused by the node’s mobility, we start by characterizing the interference caused to a receiver by the moving nodes positioned in a ring. Based on the interference distribution, we evaluate two different methodologies to estimate the interference in real-time. The accuracy of the results achieved with the proposed methodologies in several simulations show that they may be used as an effective tool of interference estimation in future wireless communication systems, being the main contribution of this work.