In the face of increasingly market competition and diversified demand, manufacturers and dealers of Manufacturing Supply Chain (MSC) pay great effort to achieve production marketing coordination. However, it often generates conflicts in the process of production marketing coordination. For this kind of conflict problem, Multi-Agent technology is introduced. Enterprises of the supply chain are represented by Agents. The conflicts among Agents are resolved through Nash negotiation. A model of Nash Negotiation based on Agents is established. The conflict point of negotiation is determined by Stackelberg differential game. A Memetic Algorithm is proposed to solve the model. Finally, the validity of the algorithm and the model is verified by numerical experiment.