【Objective】 In order to quantify the nitrogen accumulation and partitioning of protected cultivated tomato plant. 【Method】 The field experiments on cultivars and fertilizer were conducted in 2009 and 2010, and cultivars: B1, B2, and B3 were adopted.【Result】 The simulation models of nitrogen accumulation and partitioning for protected cultivated tomato plant were built by analyzing the relationship between partitioning index and product of temperature and light in accordance with the tomato growth characteristics in this paper. The models were validated using independent experiment dataset, the results showed that RMSE, Xde, and R2 between simulated and measured values of nitrogen accumulation in above-ground plant and root were 0.546 g·m− 2, 0.447 g·m− 2, and 0.956; 0.041 g· m− 2, 0.033 g·m− 2, and 0.942, respectively. That of nitrogen accumulation in stem, leaf, and fruit were 0.165 g·m− 2, 0.132 g·m− 2, and 0.839; 0.146 g·m− 2, 0.099 g·m− 2, and 0.945; 0.246 g· m− 2, 0.166 g· m− 2, and 0.845, respectively.【Conclusion】 This study indicated that the nitrogen accumulation and partitioning of different cultivars and fertilizer levels in the protected cultivated tomato plant could be well simulated by these models.